Read Mammalian Expression Vectors (pcdna3 pcdna3.1 etc)
Proteasomes generate spliced epitopes by two different
Known differences (at least): compared with NM_004964.2 ,T to C substitution at 380nt (Leu to Pro), C to T substitution at 483nt (His to His,synonym), and A to …... 1 Methods Overview Introduction pcDNA ™4/HisMax A, B, and C are 5.3 kb vectors derived from pcDNA 4/His and designed for overproduction of recombinant proteins in mammalian cell lines.
Addgene Vector Database pcDNA™3.1/myc-His(-) A
For cDNA encoding myc-(His) 6 tag, pcDNA3.1(-)-myc-His vector (Invitrogen) served as the template with primers #395 and #396. To create a cDNA encoding 3xFLAG-(His) 6, PCR was car-ried out with primers #468 and #469 in the presence of two megaprimers, #466 and #467, which served as the template. The product was digested with Xho I and Apa I and cloned into predigested pcDNA3.1(+), … life of pi part 1 summary pdf I am working with PCDNA3.1 myc-his tagged and I did transfection twice in CHO but in Western blot I had only background no specific bands.
Supplementary Materials and Methods
1 Overview Introduction pcDNAŽ3.1/myc-His(-) A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. mechanism of action of azithromycin pdf (5518) sgrD1 sspl (5077) scal (4967) (4597) Ahdl (3704) Pcil (3325) BstZ171 (3273) Bsml Pfol Created (12) Mfel (161) (180) (208) (228) Ndel (484)
How long can it take?
pcDNA 6/myc-His A B and C Thermo Fisher Scientific
- (PDF) Additional data file 3 ResearchGate
- Molecular Basis for the Differential Agonist Affinities of
- HCCR-1–Interacting Molecule “Deleted in Polyposis 1” Plays
- The E3 ubiquitin ligase CHIP selectively regulates mutant
Pcdna3 1 Myc His Pdf
Dependent on Its Receptor Interacting Protein Homotypic pEGFP-n1 and pcDNA3.1/myc/His (22, 46). Site-directed mutagenesis was performed using the QuickChange XL Site-Directed Mutagenesis Kit (Strat-agene) and conﬁrmed by sequence analysis. A series of vectors that express truncated forms of TRIF were generated by cloning PCR ampliﬁed sequences into p3XFLAG14 or pEGFP-n1, except for …
- 1 Methods Overview Introduction pcDNA ™4/HisMax A, B, and C are 5.3 kb vectors derived from pcDNA 4/His and designed for overproduction of recombinant proteins in mammalian cell lines.
- sqrD1 (5374) sspl (sasa) scal pgul (4570) Ahdl enhancer Created With Xhol (976) (12) Mfel (161) (180) Ndel (484) snaB1 Acc651 (920) gstXi EcoR1 (943)
- Then, pcDNA3.1-V5-His (Invitrogen, Carlsbad, CA) or pcDNA3.1-Myc-His was digested with XhoI to make a compatible end with SalI. A SalI fragment contain-ing the HCCR-1 or DP1 coding sequence was inserted into the XhoI-digested pcDNA3.1. Lipofectamine 2000 (Gibco BRL, Rockville, MD) was used to introduce the HCCR-1 or DP1 expression vector into HEK 293 or RKO cells. Brieﬂy, 2 105 cells were